Coding

Part:BBa_K863022:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-20)

bhal laccase from Bacillus halodurans with constitutive promoter J23100, RBS and HIS tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 219
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • PCR on BBa_K863021 with primers with BioBrick Prefix in the fwd primer and Suffix and His-tag in the rev primer.
    • fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGAAAAAATCATATGGAGT-3'
    • rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATTAGTGATGGTGATGGTGATGCTCAGGCATATTTGGAA-3'

Source

The gene sequence was isolated from Bacillus halodurans C-125.

References

Ruijssenaars, H. J. and S. Hartmans (2004). "A cloned Bacillus halodurans multicopper oxidase exhibiting alkaline laccase activity." Appl Microbiol Biotechnol 65(2): 177-182.